Experimental study of PLGA microparticles loaded with iron oxide labeling tendon stem cells in vitro
10.3760/cma.j.issn.1004-4477.2018.03.017
- VernacularTitle:载氧化铁聚乳糖-羟基乙酸微粒标记大鼠肌腱干细胞的体外实验研究
- Author:
Jingzhen JIANG
1
,
2
;
Man LU
;
Xueqing CHENG
;
Ziyue HU
Author Information
1. 637000 南充,川北医学院研究生院
2. 电子科技大学医学院附属肿瘤医院四川省肿瘤医院· 研究所四川省癌症防治中心超声医学中心
- Keywords:
Ultrasonography;
Iron oxide/PLGA microparticles;
Tendon stem cells;
Multimodal imaging;
Photoacoustic imaging;
Magnetic resonance imaging
- From:
Chinese Journal of Ultrasonography
2018;27(3):259-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare PLGA microparticles loaded with Iron oxide (PLGA/IO MPs) and explore their feasibility of the rat tendon stem cells (TSCs) labeled with the particles and the multimodal imaging of Ultrasonic (US)/Photoacoustic (PA)/Magnetic resonance (MR) in vitro. Methods The PLGA/IO MPs were prepared using double emulsification,and physical and chemical properties were tested and US/PA/MRI imaging was performed.The TSCs were labeled with PLGA/IO MPs,and transmission electron microscopy (TEM) and prussian blue staining were performed to test labeling effects,then the US, PA and MRI imaging of labeled TSCs were performed. Results The diameter and Zeta potential of prepared PLGA/IO MPs were ( 801.5 ± 165.6) nm and (6.36 ± 3.36) mV [the Zeta potential of microparticles which including poly-L-Lysine(PLL) was about (3.16 ± 3.69)mV],respectively.PLGA/IO MPs could be imaged by US/PA/MRI multimodal imaging. After labeling,the PLGA/IO MPs were distributed in cytoplasm of labeled TSCs which could be imaged by US,PA,MRI simultaneously. Conclusions The TSCs can be labeled with PLGA/IO MPs effectively,and imaged by using multimodal US/PA/MRI imaging in vitro,which will lay foundation for noninvasive and multimodal tracking of transplanted TSCs in vivo.
