Study on the therapeutic efficacy of subcutaneous pancreatic cancer xenograft tumor with ultrasound destruction of different size microbubbles
10.3760/cma.j.issn.1004-4477.2018.01.021
- VernacularTitle:超声破坏不同粒径微泡治疗皮下胰腺癌移植瘤的实验研究
- Author:
Jieli LUO
1
;
Jianshe CHEN
;
Yang SUN
;
Pintong HUANG
Author Information
1. 浙江大学医学院附属第二医院超声医学科
- Keywords:
Sonication;
Pancreatic neoplasms;
Microbubbles;
Neoplasms;
vascular tissue
- From:
Chinese Journal of Ultrasonography
2018;27(1):83-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the therapeutic efficacy of subcutaneous transplantation pancreatic cancer in nude mice model with different size microbubbles. Methods Eighteen Balb/C nude mice with subcutaneous pancreatic carcinoma xenografts were divided into three groups:group A( 6 mice) did not received microbubbles and ultrasonic irradiation;group B(6 mice) received standard microbubbles and ultrasonic cavitation treatment;group C(6 mice) received up-sized microbubbles and ultrasonic cavitation treatment.Body weight and tumor size of nude mice were measured respectively at the time points of 0,7, 14,21 days after three times ultrasound radiation and before ultrasound treatment. Tumor volumes were calculated.Then the mice were sacrificed finally. Results There was no significant difference in tumor volumes among three groups before treatment( P > 0.05). Tumor volume continued to increase in all groups.Compared with group A,growth rate in group B and group C were lower,growth rate in group B was higher than that in group C,the difference was statistically significant( P < 0.05). There was no significant difference in the parameters of regional blood volume(RBV) and regional blood flow(RBF)among three groups before treatment( P > 0.05). RBV and RBF were decreased significantly after 3 days treatment in group B and group C( P <0.05),the RBV and RBF in group C were lower than those in group B,the differences were statistically significant( P <0.05).There was no significant difference in the body weight of nude mice before and after radiation among three groups during the follow-up period( P >0.05). Conclusions The cavitation effect of up-sized microbubbles is more efficient for mice subcutaneous pancreatic cancer xenograft tumor inhibition compared with standard microbubbles.
