Comparison of Monocyte Selection Method by Immunomagnetic Adsorption or Adherence for the Generation of Dendritic cells.
- Author:
Kyung Ah CHEONG
1
;
Ok Mi CHOI
;
Sung E CHOI
;
Hyunah LEE
;
Young Joon LEE
Author Information
1. Medipost Biomedical Research Institute, Seoul, Korea. yjlee@aimmed.com
- Publication Type:Original Article
- Keywords:
dendritic cell;
immunomagnetic adsorption;
plastic adherence
- MeSH:
Adsorption*;
Cancer Vaccines;
Dendritic Cells*;
Humans;
Leukapheresis;
Monocytes*;
Plastics;
Tissue Donors
- From:Korean Journal of Blood Transfusion
2004;15(2):213-219
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Dendritic cells (DCs) are the most potent stimulators of immune response including antitumor response. DCs are currently being pursued clinically in the development of cancer vaccines; therefore there are demands for large-scale and clinical-grade generation of DCs. In the present study, to find out the most efficient separation method of DC precursors, we compared two separation methods, namely, based on magnetic based selection and plastic adherence selection. METHODS: MNCs were collected by leukapheresis from healthful donors and separated by CD14 + immunomagnetic adsorption or plastic adherence. DC precursors separated using the two methods were differenciated in the same condition. Matured DCs were compared in terms of yield, viability, the expression of surface markers and ability to induce immune reaction. RESULTS: This study demonstrated that mature DCs from CD14 + monocytes separated using CD14 + immunomagnetic adsorption had higher expression of surface markers of DCs, yield (1.9 +/-0.5% vs. 0.5 +/-0.2%), viability (94.7 +/-2.5% vs. 72.8 +/-7.5%) and better functionality in inducing immune reaction than those from plastic adherent cells. CONCLUSION: These results demonstrated that CD14 + immunomagnetic adsorption was found to be more effective than the adherent selection for the generation of DCs. This study will allow researcher to facilitate choosing the appropriate protocol to obtain DCs.
