Study on HPLC Fingerprint of Different Proportions of Angelicae Pubescentis Radix in Angelicae Sinensis Radix
10.3969/j.issn.1005-5304.2018.11.018
- VernacularTitle:当归中混入不同比例独活HPLC特征指纹图谱研究
- Author:
Ping ZHANG
1
;
Xiao MA
;
Xiao-Lin WANG
;
Dong-Hua LI
;
Ping-Shun SONG
Author Information
1. 甘肃省药品检验研究院
- Keywords:
Angelicae Sinensis Radix;
Angelicae Pubescentis Radix;
HPLC;
fingerprint;
adulteration
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(11):83-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish HPLC analysis methods and fingerprint of different proportions of Angelicae Pubescentis Radix in Angelicae Sinensis Radix; To use HPLC fingerprint method to identify the adulteration of Angelicae Pubescentis Radix in Angelicae Sinensis Radix. Methods Agilent TC-C18 column (5 μm, 4.6 mm × 250 mm) was adopted. Acetonitrile (A) - 0.05% phosphoric acid (B) was the mobile phase, and the gradient elution (0–20 min, 95%–75%B; 20–60 min, 75%–65%B; 60–80 min, 65%–5%B) was used; The flowrate was 1 mL/min; Detection wavelength was 254 nm; Column temperature was 30 ℃. The HPLC spectra of Angelicae Sinensis Radix, Angelicae Pubescentis Radix and adulterated samples were compared and analyzed by using TCM chromatogram fingerprint similarity evaluation system. Results Angelicae Sinensis Radix and Angelicae Pubescentis Radix showed good chromatographic peaks in chromatogram separation. 11 peaks in HPLC fingerprint could be used as the mark, including 7th peak for ferulic acid, 32th peak for osthole, and 34th peak for columbianadin. Conclusion This method is rapid, simple and feasible, can fully reflect the quality of Angelicae Sinensis Radix and clearly identify whether Angelicae Pubescentis Radix is adulterated in Angelicae Sinensis Radix.
