Discussion on Difference of Different Compatibility Ratios of Astragali Radix and Puerariae Lobatae Radix Based on Content Analysis
10.3969/j.issn.1005-5304.2018.11.016
- VernacularTitle:基于含量分析探讨黄芪-葛根药对不同配比的差异
- Author:
Zhi-Bin TONG
1
;
Hong TAN
;
Cong YIN
;
Lu-Ya QIU
;
Li-Jun YUAN
;
Xing TU
Author Information
1. 湖北民族学院医学院
- Keywords:
Astragali Radix and Puerariae Lobatae Radix;
compatibility ratios;
puerarin;
content analysis;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(11):73-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the contents of puerarin in decoction with different compatibility ratios of Astragali Radix and Puerariae Lobatae Radix; To verify the rationality of compatibility ratios of Huangqi Gegen Decoction and Yu y e Decoction; To provide references for clinical medication. Methods Different compatibility ratios of Astragali Radix and Puerariae Lobatae Radix were set as 0:1, 1:1, 2:1, 3:1, 4:1, 1:2, 1:3, and 1:4. SinoChrom ODS-BP (4.6 mm × 250 mm, 5 μm) was used as the chromatographic column to detect the contents of puerarin;methanol-0.1% phosphoric acid (35:65) was used as the mobile phase with 0.5 mL/min flow rate; sample volume was 20 μL; the wavelength was 250 nm. Results The regression equation of puerarin was Y=66 449.269 1X-175 665.663 1 (r=0.999 6) in the range of 5 to 300 μg/mL, showing a good linear relationship. The repeatability, stability and recovery rate were fine as well. The contents of puerarin were (2.506 7±0.025 8)%, (2.526 7±0.071 2)%, (2.863 3± 0.086 4)%, (2.956 7±0.119 6)%, (2.835 0±0.078 7)%, (2.480 0±0.072 4)%, (2.530 0±0.064 8)%, and (2.183 3±0.128 9)% in different compatibility ratios of Astragali Radix and Puerariae Lobatae Radix with 0:1, 1:1, 2:1, 3:1, 4:1, 1:2, 1:3, and 1:4, respectively. Conclusion When the compatibility ratios of Astragali Radix and Puerariae Lobatae Radix are 2:1. 3:1, and 4:1, the contents of puerarin in decoction are the highest. This study verifies that the compatibility ratios of Astragali Radix and Puerariae Lobatae Radix in classical prescriptions are rational, which can be the optimal compatibility ratios in clinic.
