Content Determination of Three Alkaloids in Aconitum Pendulum and Habitually Medicinal Materials
10.3969/j.issn.1005-5304.2018.07.020
- VernacularTitle:藏药铁棒锤及其习用品中3种生物碱含量测定
- Author:
Mei-Yu ZHAO
1
;
Liang-Ke SONG
;
Yao-Yao REN
;
Rui TAN
Author Information
1. 西南交通大学生命科学与工程学院
- Keywords:
Aconitum pendulum;
A. polyschistum;
A. sessiliflorum;
alkaloid;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(7):83-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determining benzoylaconine, aconitine and 3-deoxyaconitine in Aconitum pendulum and the habitually medicinal materials by HPLC; To provide basis for control of quality standard toxicity composition in Aconitum pendulum and difference between Aconitum pendulum and the habitually medicinal materials. Methods Agilent Eclipse XDB-C18 column (250 mm × 4.6 mm,5 μm) was used at 25 ℃ with the mobile phase consisting of acetonitrile-0.04% trimethylamine (each 1000 mL of water plus 4 mL triethylamine and 1.68 mL phosphoric acid) by gradient elution; detection wavelength was 235 nm; the flow rate was 1 mL/min; injection volume was 10 μL. Results The benzoylaconine, aconitine and 3-deoxyaconitine had good separation and linear relationship in the corresponding range (r＞0.999). The average recovery rates were 97.66%–98.47%, and RSD were 0.84%–1.60%. Conclusion The contents of 3 alkaloid were different in Aconitum pendulum and the habitually medicinal materials. Both A. polyschistum and A. sessiliflorum need separate drug names.
