Analysis of Transcriptomes and Differentially Expressed Genes in Leaves and Roots of Tetrastigma hemsleyanum Diels et Gilg.
10.3969/j.issn.1005-5304.2018.07.018
- VernacularTitle:三叶青根与茎叶转录组和差异性表达分析
- Author:
Hong-Xu LIU
1
;
Si-Shan DENG
;
Li-Hong MA
;
Xing-Quan YANG
;
Wen-Jin LIN
;
Xiao-Mei XU
Author Information
1. 福建省医学科学研究院
- Keywords:
Tetrastigma hemsleyanum Diels et Gilg.;
transcriptome;
gene;
expression difference
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(7):75-78
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain the transcriptome database and differentially expressed genes of Tetrastigma hemsleyanum Diels et Gilg. by Illumina HiSeq 4000; To provide important molecular information for its molecular biology research. Methods Leaves and roots of Tetrastigma hemsleyanum Diels et Gilg. were chosen as experimental materials to conduct transcriptome sequencing. Then bioinformatics analysis of gene function annotations, metabolic pathways, and microsatellites was performed on the test data. Results 24.13 Gb Clean Data were assembled. Afer assembly steps, 84 433 of T. hemsleyanum Unigene were obtained, and then they were compared in the 7 gene database, and 47 766 annotated information of Unigene was obtained. There were 27 790 annotations in the GO database. The number of differentially expressed genes in the roots, stems and leaves was 4989, of which 3511 were up-regulated and 1478 were down-regulated. The COG database obtained 16 152 homologous sequences of Unigene, which were divided into 25 categories. In the KEGG database, there were 14 511 Unigene obtained the corresponding Ko number, which could be divided into 130 branches of signal metabolism, among which the number of Unigene in the ribosome synthesis pathway was the most, with 1042, and there was only 1 Unigene in the biosynthetic pathway of isoflavones. Conclusion A large number of transcripts of the transcriptome were obtained through splicing, assembling and functional annotation of Tetrastigma hemsleyanum Diels et Gilg., which can provide genomic database resources for molecular biology research of Tetrastigma hemsleyanum Diels et Gilg.
