Effects of Gandouling on ROS and Nrf2 of Neural Stem Cells of Mice Cultured in High Concentration Copper
10.3969/j.issn.1005-5304.2018.07.013
- VernacularTitle:肝豆灵对高铜负荷小鼠神经干细胞活性氧水平及核因子E2相关因子2表达的影响
- Author:
Ting DONG
1
;
Wen-Ming YANG
;
Ming-Cai WU
;
Huai-Zhou JIANG
;
Peng HUANG
;
Chun-Jun KUANG
;
Juan ZHANG
;
Hui HAN
Author Information
1. 安徽中医药大学第一附属医院脑病中心
- Keywords:
Gandouling;
high copper load;
cultured in vitro;
neural stem cells;
reactive oxygen species;
nuclear factor erythroid 2-related factor 2;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(7):53-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Gandouling on reactive oxygen species (ROS) level, the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA and protein of neural stem cells of the mice cultured in high concentration copper. Methods The model of neural stem cells of the mice was cultured in vitro with high concentration copper. The experimental rats were randomly divided into blank control group, model group, and Gandouling low-, medium-, and high-dose groups. Each medication group was given relevant concentration of Gandouling serum for gavage. The MTT was adopted to test proliferation level on neural stem cells; flow cytometer was used to examine the change of ROS level in cells; qPCR was used to measure the expression of Nrf2 mRNA;Western blot was used to measure the change of the level of protein Nrf2 in cells. Results Compared with the blank control group, the proliferation rate of neural stem cells was significantly decreased, ROS levels were significantly increased, and Nrf2 gene and protein expression was significantly decreased (P＜0.01). Compared with the model group, neural stem cells proliferation rate was significantly increased, ROS levels were significantly reduced, and Nrf2 gene and protein expression was significantly increased (P＜0.05, P＜0.01). Conclusion Gandouling can promote the proliferation of neural stem cells in mice by reducing ROS content in high copper-loaded mice and up-regulating Nrf2 expression.
