Effects of Jieduan Niwan Prescription on IL-6 and TNF-α in Serum and Hepatic Tissue of Acute-on-Chronic Liver Failure Rats
10.3969/j.issn.1005-5304.2018.07.012
- VernacularTitle:截断逆挽方对慢加急性肝衰竭大鼠血清和肝组织白细胞介素-6、肿瘤坏死因子-α及肝细胞超微结构的影响
- Author:
Wei-Xin HOU
1
;
Yu-Lin HAO
;
Wen-Long YANG
;
Tian TIAN
;
Tian-Yuan JIANG
;
Qiu-Yun ZHANG
;
Lian-Yin GAO
;
Yu-Qiong DU
Author Information
1. 首都医科大学中医药学院
- Keywords:
Jieduan Niwan Prescription;
acute-on-chronic liver failure;
IL-6;
TNF-α;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(7):49-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Jieduan Niwan Prescription on IL-6 and TNF-α in serum and hepatic tissue in acute-on-chronic liver failure rats (ACLF); To study the partial mechanism of the treatment for ACLF. Methods The ACLF rat model was established by using human serum albumin immuno-induced hepatic fibrosis followed with D-galactosamine and lipopolysaccharide joint acute attack. The SPF Wistar male rats were randomly divided into normal group, model group and Jieduan Niwan Prescription group, respectively. The Jieduan Niwan Prescription group was treated by Jieduan Niwan Prescription after the acute attack for 24 h, and the rats were sacrificed respectively at 5, 10 and 15 days after gavage administration. Transmission electron microscope was employed to observe the ultrastructural changes in liver cells and ELISA was used to detect the contents of IL-6 and TNF-α in serum and liver tissue, respectively. Results Compared with the normal group, the contents of serum and liver tissue IL-6 and TNF-α in model group increased at each time point. Compared with the model group, the contents of serum and liver tissue IL-6 and TNF-α decreased in Jieduan Niwan Prescription group, especially in 15 days. Under the transmission electron microscope, the changes of ultrastructure of liver tissue were observed. With the passage of time, the degree of hepatocyte injury in model group gradually increased, but decreased significantly in Jieduan Niwan Prescription group at each time point. Conclusion Jieduan Niwan Prescription can effectively reduce the levels of IL-6 and TNF-α in serum and liver tissue, reduce the degree of liver damage, and has a certain protective effect on the liver.
