Research on HPLC Fingerprint of Jinsangqi Kangdu Dropping Pills
10.3969/j.issn.1005-5304.2018.06.022
- VernacularTitle:金桑芪抗毒滴丸HPLC特征指纹图谱研究
- Author:
Hong-Yan LIU
1
;
Jing WU
;
Li-Na LIU
;
Zhong JIA
Author Information
1. 兰州市肺科医院
- Keywords:
Jinsangqi Kangdu Dropping Pills;
calycosin-7-O-β-D-glucopyranoside;
rutin;
liquiritin;
hyperoside;
quercetin;
ammonium glycyrrhizinate;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(6):90-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish fingerprint of Jinsangqi Kangdu Dropping Pills by HPLC; To control the quality of the preparation. Methods Waters XSELECT CSH-C18 chromatographic column (4.6 mm × 150 mm, 5 μm) was used and eluted with acetonitrile - 0.1% phosphoric acid solution gradient at the flow rate of 1.0 mL/min. The detection wavelength was 260 nm with column temperature of 30 ℃. Using calycosin-7-O-β-D-glucopyranoside, rutin, liquiritin, hyperoside, quercetin and ammonium glycyrrhizinate as the object references, ten batches of Jinsangqi Kangdu Dropping Pills were tested and analyzed by similarity comparison.Results Fringerprint spectrum of Jinsangqi Kangdu Dropping Pills had 24 common peaks in total, and characteristic spectrums of Hypericum Perforatum, Mori Cortex, Astragali Radix and Glycyrrhizae Radix et Rhizoma had been found, while similarity of HPLC fingerprint was more than 0.9 among those batches of samples. Conclusion Using HPLC fingerprint can evaluate the Jinsangqi Kangdu Dropping Pills quality totality,which can provide references for improving the quality control of the preparation.
