Qualitative and Quantitative Analysis of Active Components of Copditis Rhizoma in Gandouling Pills by HPLC
10.3969/j.issn.1005-5304.2018.06.021
- VernacularTitle:肝豆灵片中黄连有效成分HPLC定性定量分析
- Author:
Li CHEN
1
;
Sheng WANG
;
Xiu-Juan QIN
;
Mei MENG
Author Information
1. 安徽中医药大学第一附属医院
- Keywords:
Gandouling Pills;
active components of Coptidis Rhizoma;
HPLC;
qualitative analysis;
quantitative analysis
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(6):87-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the HPLC conditions for active components of Coptidis Rhizoma; To establish qualitative and quantitative analysis method for related components of Coptidis Rhizoma in Gandouling Pills. Methods HPLC was used for the qualitative and quantitative analysis of related components of Coptidis Rhizoma in Gandouling Pills.With acetonitrile:0.05 mol/L potassium dihydrogen phosphate solution=50:50(per 100 mL was added 0.4 g sodium dodecyl sulfate, and then adjusted pH to 4.0 with phosphoric acid) as mobile phase, the flow rate was 1.0 mL/min and the detection wavelength was 345 nm. Results Four components of Coptidis Rhizoma can be identified, including berberine, coptisine, berberine hydrochloride and palmatine chloride, and the negative control had no disturbance. Berberine hydrochloride reference and the absorbance peak area value showed a good linear relationship within 0.058-0.580 μg sample volume, and palmatine chloride reference was the same within 0.030-0.300 μg sample volume. The average recovery was 98.18% and 99.99%, and RSD was 2.40% and 1.70%, respectively. Conclusion The method is simple, accurate and with good repeatability, which can be applied effectively to the quality control of Gandouling Pills.
