Content Determination of Total Flavonoids and Shikimic Acid in Pine Needles of Pinus massoniana Lamb. in Wudang Area
10.3969/j.issn.1005-5304.2018.06.017
- VernacularTitle:武当地区马尾松松针中总黄酮及莽草酸含量测定
- Author:
Chang-Hu KE
1
;
Fang ZHENG
;
Hui YAN
;
Hui-Min LIU
;
Pei-Hu FU
;
Ping HU
;
Xue-Song ZHU
Author Information
1. 湖北医药学院附属东风医院
- Keywords:
Pinus massoniana Lamb.;
pine needles;
total flavonoids;
shikimic acid;
content determination;
UV spectrometry;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(6):69-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method to determine the contents of total flavonoids and shikimic acid in pine needles of Pinus massoniana Lamb.in Wudang Area.Methods Rutin was used as reference standard,and the content of total flavonoids in pine needles of Pinus massoniana Lamb. was determined by UV spectrometry at wavelength of 500 nm. The content of shikimic acid was determined by HPLC-DAD. The Fortis Xi C8 column (5 μm, 250 mm × 4.6 mm) was adopted with acetonitrile - 0.4% phosphoric acid solution (8:92, V/V) as mobile phase at the flow rate of 0.9 mL/min. The detection wavelength was 213 nm; the column temperature was 30 ℃; the injection volume was 20 μL. Results The linear range was 8.26-49.54 μg for rutin (r=0.999 4) with an average recovery of 99.2%, RSD=1.94%. The linear range was 10.26-61.56 μg for shikimic acid (r=0.999 4) with an average recovery of 99.5%,RSD=1.93%.The contents of total flavonoids in pine needles of Pinus massoniana Lamb.was 28.33 mg/g, and shikimic acid was 15.25 mg/g, respectively. Conclusion The method is simple, rapid, accurate and reproducible. It can be used for the content determination of total flavonoids and shikimic acid in pine needles of Pinus massoniana Lamb. in Wudang Area.
