Effects of Jianpi Xiaoai Prescription on Cell Cycle and Apoptosis of Human Colon Cancer HCT116 Cells and Wnt/β-catenin Signaling Pathway Related Proteins
10.3969/j.issn.1005-5304.2018.06.015
- VernacularTitle:健脾消癌方对人结肠癌HCT116细胞周期、凋亡及Wnt/β-catenin信号通路相关因子的影响
- Author:
Xiao YANG
1
;
Wei TANG
;
Yi-Lan JIANG
;
Zi-Hao TAO
;
Ke LIU
;
Cheng SONG
Author Information
1. 湖南中医药大学研究生院
- Keywords:
Jianpi Xiaoai Prescription;
colorectal cancer;
Wnt/β-catenin signaling pathway;
apoptosis;
cell cycle
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(6):61-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Jianpi Xiaoai Prescription on cell cycle and apoptosis of human colon cancer HCT116 cells and related factors of Wnt/β-catenin signaling pathway; To investigate its mechanisms of anti-metastasis and recurrence of colorectal cancer. Methods The logarithmic growth phase HCT116 cells were divided into blank group, saline group, 5-Fu group, and Jianpi Xiaoai Prescription low-, medium-, and high-dose groups. After intervention for 48 h, the cells were harvested, and the cell cycle and apoptosis were detected by flow cytometry. The protein expression of β-catenin in the nucleus was detected by Western blot, and the expression of c-myc and cyclinD1 mRNA was detected by PCR. Results Compared with the blank group and saline group, the ratio of HCT116 cells apoptosis of Jianpi Xiaoai Prescription low-, medium-, and high-dose groups increased; the proportion of cells in phase G1 increased and the proportion of S cells decreased; the expression of β-catenin protein in the nucleus and the expression of c-myc,cyclinD1 mRNA decreased,especially in the Jianpi Xiaoai Prescription high-dose group,with statistical significance(P<0.05,P<0.01).Conclusion Jianpi Xiaoai Prescription can promote apoptosis of human colon cancer HCT116 cells and block the cell cycle, and its mechanism may be related to regulating Wnt/β-catenin signaling pathway.
