Effects of Acupuncture on Inflammation in Prefrontal Cortex of Vascular Dementia Rats
10.3969/j.issn.1005-5304.2018.06.012
- VernacularTitle:针刺对血管性痴呆大鼠前额叶皮层炎症水平的影响
- Author:
Jing-Wen YANG
1
;
Li-Ting LIN
;
Tian-Ran LI
;
Shuai ZHANG
;
Xue-Rui WANG
;
Cun-Zhi LIU
Author Information
1. 首都医科大学附属北京中医医院
- Keywords:
vascular dementia;
acupuncture;
microgila;
TLR4;
inflammation;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(6):48-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of acupuncture on TLR4, inflammation factors IL-1β and IL-6 expressions in the prefrontal cortex of vascular dementia (VD) rats; To investigate the brain protective mechanisms of acupuncture. Methods A total of 50 rats were randomly divided into sham-operation group, model group, acupuncture group, and placebo-acupuncture group. The animal model of VD was replicated by permanent bilateral common carotid artery occlusion (2VO) in rats. Acupuncture was performed at three days after surgery, once daily for two weeks. Morris water maze was used to test the cognitive function. The expressions of Iba1 and TLR4 were assessed by immunohistochemical method. Inflammation factors IL-1β and IL-6 mRNA expressions were tested by RT-PCR. Results Compared with sham-operation group, the model group rats showed impaired spatial learning and memory ability, and expression of Iba1, TLR4, and IL-1β, IL-6 increased. Compared with the model group, the spatial learning and memory abilities of the acupuncture group were improved, and the expression of Iba1, TLR4 and IL-1β, IL-6 in the prefrontal cortex decreased. These effects were not found in the placebo-acupuncture group. Conclusion The effect of acupuncture may be achieved by inhibiting microglia activate, decreasing the expressions of TLR4, IL-1β and IL-6 in the prefrontal cortex, resulting in improving spatial learning and memory ability of VD rats.
