Study on Qualitative and Quantitative Analysis of Zhendian Kaiqiao Granules
10.3969/j.issn.1005-5304.2018.04.016
- VernacularTitle:镇癫开窍颗粒定性定量方法研究
- Author:
Rui-Tang ZHANG
1
;
Yong-Xin JING
;
Hong-Mei ZHANG
;
Xiao MA
;
Cai-Hong HE
;
Xin-Hua TIAN
;
Yu-Qiong WU
Author Information
1. 西北民族大学
- Keywords:
Zhendian Kaiqiao Granules;
gentiopicrin;
paeoniflorin;
Gentiane Radix et Rhizoma;
TLC identification;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(4):77-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for qualitative and quantitative analysis of Zhendian Kaiqiao Granules. Methods Gentiane Radix et Rhizoma, Scuteliariae Radix, Rhei Radix et Rhizoma, Radices Paeoniae Alba in the preparation were identified by TLC. Gentiopicrin and paeoniflorin were determined by HPLC. The analysis was performed on a Phenomenex C18 column (250 mm × 4.6 mm, 5 μm), with the mobile phase of methyl alcohol-water (12:88). The flow rate was 1.0 mL/min and the column temperature was maintained at room temperature; the detection wavelengths were set at 273 nm (gentiopicrin) and 230 nm (paeoniflorin). Results Gentiane Radix et Rhizoma, Scuteliariae Radix, Rhei Radix et Rhizoma, Radices Paeoniae Alba could be detected by TLC. Gentiopicrin showed a good linear relationship at a range of 2.396–11.980 μg, r=0.999 6. The average recovery was 99.72%, and RSD was 2.70%. Paeoniflorin showed a good linear relationship at a range of 2.728–13.640 μg, r=0.999 0. The average recovery was 98.74%, and RSD was 2.42%. Conclusion The established method is simple, reliable and reproductive.The method can be used for control quality of Zhendian Kaiqiao Granules.
