Content Determination of 5 Anthraquinones in Huangzhi Neiyi Capsules by QAMS
10.3969/j.issn.1005-5304.2018.04.014
- VernacularTitle:一测多评法测定黄蛭内异胶囊中5种蒽醌类成分含量
- Author:
Chong XU
1
;
Jie SHEN
;
Min XIA
;
Min YANG
;
Jing LENG
Author Information
1. 重庆市中医研究院古验方与中药制剂研究所
- Keywords:
quantitative analysis of multi-component by single-marker;
Huangzhi Neiyi Capsules;
anthraquinones;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(4):66-70
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the contents of 5 anthraquinones (aloe-emodin, phein, emodin, chrysophanol, physcion)in Huangzhi Neiyi Capsule through a quantitative analysis of multi-component by single-marker(QAMS), To determine the application value of QAMS by comparing with external standard method (ESM). Methods HPLC was performed at 30 ℃ with Waters Symmetry C18 column (4.6 mm × 250 mm, 5 μm), methanol-0.4% phosphoric acid (85:15) as mobile phase at the wavelength 440 nm, and flow rate of 1.000 mL/min. The relative correction factors of other anthraquinones were calculated respectively by emodin as the reference. Results The values calculated by QAMS were compared with the values measured by ESM, and no significant difference was showed (RSD<5%). Conclusion For the availability and reliability, QAMS can be applied for quality evaluation of Huangzhi Neiyi Capsules.