Effects of Acupuncture on the Level of Inflammatory Cytokines and Toll-like Receptor 4 in Hypothalamic Paraventricular Nucleus of Spontaneously Hypertensive Rats
10.3969/j.issn.1005-5304.2018.04.013
- VernacularTitle:针刺对自发性高血压大鼠下丘脑室旁核炎症因子含量及Toll样受体4表达的影响
- Author:
Cai-Shuo JI
1
;
Si-Ming MA
;
Na-Na YANG
;
Zhong-Xue TIAN
;
Jing-Wen YANG
;
Jia-Kai SHAO
;
Xue-Rui WANG
;
Cun-Zhi LIU
Author Information
1. 首都医科大学附属北京中医医院针灸中心
- Keywords:
acupuncture;
Taichong;
high pressure;
TLR4;
inflammatory cytokines;
spontaneously hypertensive rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(4):62-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of acupuncture on the level of inflammatory cytokines and toll-like receptor 4 in hypothalamic paraventricular nucleus (PVN) of SHR; To investigate the mechanisms of acupuncture in reduction of mean arterial blood pressure (MAP) in SHR. Methods Thirty 10-week old SHR were randomly divided into SHR group, acupuncture group and non-acupoint group, with 10 rats in each group. 10 WKY rats were set as control group. Acupuncture group received bilateral acupuncture in "Taichong" acupoint, and twisting and diarrhea method was used to stimulate; non-acupoint group received acupuncture at the back of feet, and soothing and diarrhea with twisting method was used to stimulate. Materials were taken two weeks later. The mean arterial pressure of rats was detected every day; the expression of TLR4 mRNA in PVN was detected by RT-PCR; The expression of TLR4 protein in PVN was detected by Western blot; The levels of TNF-αa nd IL-6 were detected. Results Compared with control group, the mean arterial pressure of SHR group increased; TLR4 mRNA and protein expression in PVN increased significantly; the level of TNF-α and IL-6 increased significantly (P<0.05). Compared with SHR group, the mean arterial pressure of acupuncture group decreased significantly;TLR4 mRNA and protein expression in PVN decreased significantly; the level of TNF-α and IL-6 decreased significantly (P<0.05). There was no significant change in non-acupoint group. Conclusion Acupuncture in"Taichong" acupoint can attenuate blood pressure of SHR by inhibiting expression of TLR4 in PVN and reducing the levels of TNF-α and IL-6.
