Isolation and Content Determination of Hydrangenol in Leaves of Hedyotis Hedyotidea (DC) Merr by HPLC
10.3969/j.issn.1005-5304.2018.03.021
- VernacularTitle:牛白藤叶中绣球酚的分离及含量测定
- Author:
Zhen-Hai YUAN
1
;
Yan LIU
;
Li-Xia SHANG
;
Hong-Yan WANG
Author Information
1. 山东省药学科学院
- Keywords:
Hedyotis hedyotidea(DC)Merr;
hydrangenol;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(3):94-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To conduct isolation identification of hydrangenol and establish a method for content determination of hydrangenol in the leaves of Hedyotis hedyotidea (DC) Merr; To provide references for further development and research of Hedyotis hedyotidea;To compare the contents of hydrangenol in the leaves of Hedyotis hedyotidea of different production areas and different batches. Methods Hydrangenol showed a good linear relationship within the range of 0.412–10.300 μg (r=1.000 0), with the average recovery of 99.09% (RSD=0.53%). Good precision and repeatability were achieved with the RSDs smaller than 1.0%. The content range was 0.03%–0.38% for hydrangenol in the leaves of Hedyotis hedyotidea of different production areas and different batches, and the highest contents appeared in those from Guangxi. Conclusion The method is simple, accurate, specific, reproducible and can be used for the content determination of hydrangenol in the leaves of Hedyotis hedyotidea; the contents of hydrangenol in the leaves of Hedyotis hedyotidea of different production areas and different batches are different, and contents in samples from Guangxi and Guangdong are higher.
