Pharmacokinetic Features of Reactive Sulfide in Plasma of Rats After Oral Administration of Cinnabaris by Using HPLC Coupled with Pre-column Derivatization
10.3969/j.issn.1005-5304.2018.03.019
- VernacularTitle:柱前衍生化-高效液相色谱法测定大鼠灌服朱砂后血浆中活性硫的药动学特征
- Author:
Tao-Guang HUO
1
;
Jing-Tan GUO
;
Ying-Hua ZHANG
;
Qiu LIU
;
Zhong-Xing FU
;
Hong JIANG
;
Ting-Guo KANG
Author Information
1. 中国医科大学公共卫生学院
- Keywords:
Cinnabaris;
reactive sulfide;
precolumn derivatization-HPLC;
pharmacokinetic features
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(3):86-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the pharmacokinetic features of reactive sulfide in rats after oral administration of Cinnabaris. Methods An HPLC coupled with precolumn derivatization method was developed for the pharmacokinetic features study on reactive sulfide in rats after oral administration of Cinnabaris. Results Good linearity (r>0.99) was found for reactive sulfide in plasma in the concentration range of 0.25–15 μmol/L (r>0.99). The LOQ and LOD of the method were 0.1 μmol/L and 0.02 μmol/L, respectively. The intra- and inter-day precision was less than 4.4% and 3.5% respectively, and the accuracy was -9.9%–6.0%. The average recovery rate was 74.9%. 0.6 g/kg Cinnabaris was given the rats for gavage, and the time-course pharmacokinetics parameters were as follows:Cmax(1.33±0.13) μmol/L, tmax(150±34) min, t1/2(323±62) min, AUC0-∞ (5743±297) ng/mL?h. Conclusion A sensitive, robust and accurate precolumn derivatization-HPLC method for the determination of plasma reactive sulfide is developed and validated. The method is successfully applied in the pharmacokinetic features study on reactive sulfide in plasma of rats after administration of Cinnabaris.
