Study on Cellular Uptake Mechanism of HepG2.2.15 Cells to Nanoparticles Co-loaded with Syringopicroside and Hydroxytyrosol
10.3969/j.issn.1005-5304.2018.03.018
- VernacularTitle:HepG2.2.15细胞对同时包载丁香苦苷和羟基酪醇纳米粒的摄取机制研究
- Author:
Qing-Xia GUAN
1
;
Yun-Xing LI
;
Shao-Wa LV
;
Jia-Lin SUN
;
Liang ZHANG
;
Wen-Jing FENG
;
Li-Ping WANG
;
Yong-Ji LI
Author Information
1. 黑龙江中医药大学
- Keywords:
syringopicroside;
hydroxytyrosol;
mPEG-PLGA nanoparticles;
HepG2.2.15 cells;
uptake mechanism
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(3):81-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the uptake mechanism of HepG2.2.15 cells to the nanoparticles co-loaded with syringopicroside and hydroxytyrosol (SH-NPs). Methods The nanoparticles were prepared by using a nanoprecipitation method with mPEG-PLGA as nano-carrier co-loaded with syringopicroside and hydroxytyrosol. The uptake mechanism of HepG2.2.15 cells to SH-NPs was studied by fluorescence microscopy and flow cytometry using fluoresceineisothiocyanate (FITC) as a fluorescent marker. Results With colchicine as the inhibitor, the incubation time ranged from 0.5 to 24 h, the percentage of positive cells increased from 1.9% to 56.4%; When the drug concentration was 125, 250 μg/mL and 500 μg/mL, the positive cell percentages were 4.9%, 3.4% and 3.9%. With chloroquine as the inhibitor; the incubation time ranged from 0.5 to 24 h, the percentage of positive cells increased from 7.4% to 55.4%; When the drug concentration was 125, 250 and 500 μg/mL, the percentage of positive cells was 19.5%, 22.5% and 27.6%. Conclusion Colchicine and chloroquine have an inhibitory effect on HepG2.2.15 cells uptake, and the uptake of SH-NPs in HepG2.2.15 cells was positively correlated with drug concentration and incubation time. It can be concluded that the uptake mechanism of HepG2.2.15 cells to SH-NPs was nonspecific adsorption endocytosis.
