Effects of Warming Acupuncture Therapy on Expressions of IL-6 and SOCS3 in Spinal Cord in Rats with Neuropathic Pain
10.3969/j.issn.1005-5304.2018.03.014
- VernacularTitle:温针灸对神经痛大鼠脊髓白细胞介素-6及细胞信号转导抑制因子3的影响
- Author:
Xiang-Mei YU
1
;
Yi-Zhou YANG
;
Jian-Bo LIU
;
Chang-Zheng LI
;
De-Gui GONG
;
Zhi-Fu WANG
Author Information
1. 福建中医药大学中西医结合学院
- Keywords:
neuropathic pain;
warming acupuncture therapy;
spinal cord;
IL-6;
SOCS3;
nerve inflammation;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(3):64-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effects of warming acupuncture therapy on expressions of IL-6 and SOCS3 in spinal cord in rats with neuropathic pain; To discuss its mechanism for treating neuropathic pain. Methods Experimental rats were randomly divided into: normal group, model group, warming acupuncture and IL-6 group, with 6 rats in each group. Sciatic nerve chronic constriction injury neuralgia model was established in the model group, without intervention. After modeling for 5 days in the warming acupuncture group, "Pishu" and "Shenshu" acupoints were chosen for warming acupuncture therapy for 10 times. After modeling for 5 days in the IL-6 group, IL-6 group was successfully intrathecally injected 3 times with recombinant IL-6. After finishing all experiments, the mechanical pain behavior was measured with electronic Frye fibers. The mRNA levels of IL-6 and SOCS3 and protein concentration of spinal Iba-1were detected with ELISA and RT-PCR analysis. Results Compared with model group, mechanical withdrawal thresholdsin the warm acupuncture group significantly increased, and the content of Iba-1 decreased significantly (P<0.01); The mRNA level of IL-6 decreased significantly (P<0.01), and the mRNA level of SOCS3 significantly increased (P<0.01). Conclusion Warming acupuncture therapy can reduce the pain response in rats with neuropathic pain through inhibiting spinal cord microglial activation, down-regulating the gene expression of lL-6 and up-regulating the gene expression of SOCS3.
