Effects of Bushen Huoxue Acupuncture Method on Proteomics Expression in Amygdaloid Tissue of SAMP8
10.3969/j.issn.1005-5304.2018.03.013
- VernacularTitle:补肾活血针刺法对SAMP8小鼠杏仁核蛋白质组学表达的影响
- Author:
Mei-Ting LIANG
1
;
Hong ZHU
;
Ke-Li DONG
;
Guang-Cheng LI
Author Information
1. 中南大学湘雅三医院
- Keywords:
Alzheimer disease;
Bushen Huoxue;
acupuncture method;
SAMP8 mice;
amygdala;
proteomics
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(3):58-63
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Bushen Huoxue acupuncture method on amygdaloid protein expression in SAMP8; To explore the potential target protein for acupuncture treatment of Alzheimer disease (AD). Methods Thirty six-month-old male SAMP8 mice were randomly divided into acupuncture group and control group, 15 mice in each group. Acupuncture group selected Baihui (GV20), Shenshu (BL23), Geshu (BL17) and Xuehai (SP10) to intervene. The control group was given the same time to catch and stimulate. After 8 weeks, the amygdala was extracted and the differential expression protein spots were identified by proteomic techniques. Results Compared with control group, acupuncture group eventually identified 9 differential expression protein spots, of which 6 up-regulated and 3 down-regulated. According to the relevant information provided in the protein database, the main function of differential expression proteins involved in the mitochondrial energy metabolism, oxidative stress, and production of Aβ. Conclusion Bushen Huoxue acupuncture method can regulate multiple protein expressions in amygdala, suggesting that it may be through improving mitochondrial energy metabolism, oxidative stress, reducing production of Aβ to realize the potential therapeutic effects on AD.
