Study of Acute Toxicity Different Extraction Parts of Aconitum sinomontanum Nakai on Mice
10.3969/j.issn.1005-5304.2018.03.012
- VernacularTitle:高乌头不同溶剂萃取部分对小鼠的急性毒性研究
- Author:
Li-Jun ZHANG
1
;
Yun LI
;
Yan-Xia ZHANG
;
Zhi-Jun YANG
;
Jun MA
;
Hai-Rong DAI
Author Information
1. 甘肃中医药大学
- Keywords:
Aconitum sinomontanum Nakai;
different extraction parts;
acute toxicity;
LD50;
maximum dose;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(3):53-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the toxicity of different extraction parts of Aconitum sinomontanum Nakai;To screen out"toxic sections"of Aconitum sinomontanum Nakai; To provide references for further study on toxicity components of Aconitum sinomontanum Nakai. Methods Systematic solvent method was used to extract the 95% ethanol extracts of Aconitum sinomontanum Nakai,and six different extraction fractions(petroleum ether,chloroform, ethyl acetate, butanol, alcohol and water) were obtained. Median lethal dose (LD50) and maximum dose method were used to conduct comparative study on acute toxicity of different extraction parts of Aconitum sinomontanum Nakai. Results Chloroform, water and butanol extractions in LD50of Aconitum sinomontanum Nakai were 89.65, 1805.40 and 24 409.41 mg/kg, and 95% confidence limits were 76.39~108.41, 1521.60~2240.00 and 20 422.54~24 246.95, respectively. The maximum dose of petroleum ether, ethyl acetate and alcohol extractions were 2686.01, 3108.13 and 28 376.21 mg/kg, respectively. Conclusion The maximum toxicity is the extracted section of chloroform, and the minimal toxicity is the extracted section of ethanol.
