Study on Response of TRPV1 Sensitive Neurons in Spinal Cord under Moxibustion of Different Temperatures
10.3969/j.issn.1005-5304.2018.03.011
- VernacularTitle:脊髓背角瞬时感受器电位香草酸受体1敏感性神经元对不同温度艾灸响应的研究
- Author:
Hui CAI
1
;
Ming WANG
;
Chuan-Yang JIN
;
Zhi YU
;
Xin-Jun WANG
;
Jian-Bin ZHANG
Author Information
1. 南京中医药大学第二临床医学院
- Keywords:
spinal dorsal cell;
neuron;
moxibustion;
Zusanli;
temperature;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(3):48-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the response of TRPV1 sensitive neurons in spinal cord under moxibustion of different temperatures in "Zusanli". Methods Totally 40 healthy male SD rats were chosen and stimulated with moxibustion at "Zusanli" in 41 ℃, 45 ℃ and 49 ℃. Extracellular recording techniques of glass microelectrode was used in the rats' L4spinal cord neurons. Capsaicin vehicle and solution was injected into the rats through "Zusanli" to filter the sensitive neuron to TRPV1. The response of sensitive neurons to TRPV1 in spinal cord under moxibustion of three different temperatures was recorded and compared. Results The TRPV1 sensitive neurons responded to all the three temperatures. Viewing from the nature of the responding neurons, the neuron in 45 ℃ and 49 ℃ mainly performed in agitation, while the number of agitated and inhibitory neurons appeared same in 41 ℃. Viewing from the character of the responding neurons, different temperatures correspond to differential discharge frequency: 41 ℃was (8.26±2.14) spikes/s, 45 ℃ was (4.69±2.61) spikes/s, 49 ℃ was (10.66±5.97) spikes/s. Viewing from the change of discharge frequency, the neurons in 41 ℃ was found without obvious change, while the neurons in 45 ℃and 49 ℃ discharge frequency apparently increased (P<0.01). Conclusion Sensitive neurons in TRPV1 in spinal cord can response to 41 ℃, 45 ℃ and 49 ℃, whose character, nature and degree are related to the temperature gradient.
