Fast Identification Analysis on Chemical Constituents in Mori Folium by Using UPLC-Q-TOF/MS Method
10.3969/j.issn.1005-5304.2018.02.016
- VernacularTitle:基于UPLC-Q-TOF/MS技术的桑叶化学成分快速识别分析
- Author:
Jin-Ling LIU
1
;
Wen-Jiao LI
;
Ren WANG
;
Sen LI
;
Yu-Tian ZHANG
;
Jin ZHOU
;
Fu-Yuan HE
Author Information
1. 湖南中医药大学药学院
- Keywords:
Mori Folium;
UPLC-Q-TOF-MS;
chemical constituents;
flavonoids
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(2):69-73
- CountryChina
- Language:Chinese
-
Abstract:
Obejective To conduct fast identification analysis on chemical constituents of Mori Folium UPLC-Q-TOF/MS method.Methods ACQUITY UPLC-Q-TOF/MS spectrometer was used, acetonitrile and water (containing 0.1％ formic acid) as mobile phase with gradient elution. The flow rate was 0.3 mL/min, with ESI negative ion mode detection, data analysis with Masslynx4.1 software.Results According to the retention time provided by the mass spectrometry, the exact molecular weight and the secondary mass spectrometry were used to analyze the fragments by referring to literature, and 19 chemical constituents of Mori Folium were identified and deduced.Conclusion The method can analyze the chemical constituents of Mori Folium rapidly, sensitively and comprehensively, and provide the basis for the study of the pharmacological basis of Mori Folium.
