Expression and diagnostic value of serum miR-26a/b in patients with gastric cancer
10.3969/j.issn.1000-8179.2017.24.612
- VernacularTitle:胃癌患者血清miR-26a/b的表达水平及诊断价值
- Author:
Yiran SI
1
;
Haiyang ZHANG
;
Yi WANG
;
Ting DENG
;
Ming BAI
;
Tao NING
;
Le ZHANG
;
Yi BA
Author Information
1. 天津医科大学肿瘤医院消化肿瘤内科
- Keywords:
gastric cancer;
serum miR-26a/b;
clinicopathologic features;
diagnostic value
- From:
Chinese Journal of Clinical Oncology
2017;44(24):1242-1247
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the diagnostic value of miR-26a/b and relationship of this microRNA with clinicopathological features in patients with gastric cancer. Methods:The expression of serum miR-26a/b was detected in 121 patients with gastric cancer and 116 healthy controls using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The relationship of miR-26a/b and clinicopathological parameters of patients were analyzed. The receiver operating curve (ROC) was constructed to in vestigate the value of miR-26a/b in the diagnosis of gastric cancer. Results:The relative expression levels of serum miR-26a and miR-26b in patients with gastric cancer were lower than those in the control group (1.60±1.02 vs. 5.35±0.44;1.44±0.71 vs. 5.35±0.71;P<0.05). The relative expression level of miR-26a/b correlated with TNM stage, and the relative expression level of miR-26a also correlated with invasion depth and lymph node metastasis (P<0.05), but not with sex, age, tumor size, or histological type (P>0.05). The area under the ROC curve of miR-26a was 0.828 (95％CI:0.776～0.881), with sensitivity of 73.3％and specificity of 81.0％, while the area under the ROC curve of miR-26b was 0.853 (95％CI:0. 801～0.906), with sensitivity of 68.1％and specificity of 99.2％. Conclusion:The expression of miR-26a/b significantly reduced in patients with gastric cancer, and its expression level correlated with the clinical stage, invasion depth, and lymph node metastasis. MiR-26a/b has potential diagnostic value for gastric cancer.
