MUC1 Mucin 1 Antibody Marked Superparamagnetic Iron Oxide Nanoparticles in Nude Mice Model of Pancreatic Cancer: An Experimental Study
10.3969/j.issn.1005-5185.2017.10.001
- VernacularTitle:MUC1黏蛋白1抗体标记超顺磁纳米颗粒在胰腺癌裸鼠模型中的实验研究
- Author:
Zhongjie ZHANG
1
;
Qi ZOU
;
Jie CHEN
;
Chunsheng LI
Author Information
1. 复旦大学附属浦东医院肝胆外科 上海201399
- Keywords:
Pancreatic neoplasms;
Magnetic resonance imaging;
Mucins;
Molecular probes;
Nanospheres;
Iron compounds;
Contrast media;
Disease models,animal;
Mice,nude
- From:
Chinese Journal of Medical Imaging
2017;25(10):721-725
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To prepare superparamagnetic iron oxide nanoparticles (SPION) probe targeted and modified by MUC1 murin (MUC1) in order to explore its MRI characteristics in pancreatic cancer transplantation model.Materials and Methods Chemical conjugate method was adopted for coupled response of MUC1 and SPION to construct targeted probe and tested its basic physical properties,including water and diameter,surface charge and MR signal measuring.Meanwhile,nude mice model of pancreatic cancer transplant subcutaneous sarcoma was set up to study imaging effect inside the nude mice.Transplant sarcoma specimen was taken and immunohistochemical and Western blot were adopted to measure MUC1 expression.Results Partial size of the prepared particle probe was approximately 63.5 nm and surface charge was about 10.2 mV.The probe solution could obviously decrease MR transverse relaxation time (T2 value).In vitro experiment,MUC 1 could selectively gather on nude mice transplant sarcoma model could greatly lower T2 signal intensity.Conclusion Prepared probe has small partial size,superparamagnetic and other advantages.It can realize combination with pancreatic cancer tissue specificity and provide reliable in vivo iconology in early stage for disease diagnosis through vitro imaging.
