Low frequency ultrasound combined with microbubbles and brain-derived neurotrophic factor drugs in prevention of ischemic stroke in rats
10.13929/j.1003-3289.201705151
- VernacularTitle:低频超声联合微泡及脑源性神经营养因子预防治疗大鼠缺血性卒中
- Author:
Yi WANG
1
;
Ling WANG
;
Shaojun KANG
;
Gui ZENG
Author Information
1. 重钢总医院神经外科
- Keywords:
Infarction,middle cerebral artery;
Brain-derived neurotrophic factor;
Blood brain barrier;
Ultrasonography
- From:
Chinese Journal of Medical Imaging Technology
2018;34(1):15-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the preventive therapeutic effects of brain-derived neurotrophic factor (BDNF) on acute ischemic stroke in rats under ultrasound combined with microbubbles.Methods Twenty-seven healthy SD rats were randomly divided into sham-operation group (n=9),BDNF group (n=9) and BDNF plus ultrasound group (n=9),then middle cerebral artery occlusion (MCAO) models were established.The concentration of BDNF in targeted brain tissue was measured at 24 h after operation.MRI examination and behavioral assessment were performed at 1,3 and 7 days after operation.Results The concentration of BDNF in BDNF plus ultrasound group was significantly higher than that in BDNF group and sham operation group (all P＜0.05).The infarct size in BDNF group gradually reduced with time increase,and the infarct size was significantly smaller than that of BDNF group at the 7th day.The behavioral assessment had a significant statistical difference between BDNF plus ultrasound group and BDNF group at the 7th day after the operation (P＜0.05).Conclusion The preventive therapeutic effect of BDNF could be enhanced by ultrasound in the rat model with acute ischemic stroke,which can significantly reduce the size of the infarct and improve the behavior of the rats.
