Protective effects of shenfu injection on cerebral ischemia reperfusion injury in rats
10.3760/cma.j.issn.1008-1372.2018.10.012
- VernacularTitle:参附注射液对大鼠脑缺血再灌注损伤的保护作用研究
- Author:
Changxi LI
1
;
Lifeng WU
;
Guirong ZENG
;
Zhengang SHI
;
Xiangzhong YUAN
;
Yongxing XU
;
Miaohong ZHANG
Author Information
1. 湖南省药品审评认证与不良反应监测中心
- Keywords:
SHENFU DECOCTION/TU;
Ischemic preconditioning;
Brain ischemia;
Reperfusion injury/DT;
Rats
- From:
Journal of Chinese Physician
2018;20(10):1487-1490
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of shenfu injection on cerebral ischemia reperfusion injury in rats.Methods 120 male Sprague Dawley (SD) rats(320-350 g) were randomly divided into sham operation group,model control group,Nimodipine injection group,low,medium and high dose group of shenfu injection according to gender weight.20 males in each group were given medicine once a day for 7 days before operation.The cerebral ischemia model was established by thread embolization after 5 days of administration.In the sham operation group,the other operations were the same as those in the model group except for carotid artery ligation and thread insertion.After 24 hours of perfusion,the neurological score,abdominal aorta blood flow,malondialdehyde (MDA),superoxide dismutase (SOD) and glutathione (GHS) levels in brain tissues were measured.Triphenyltetrazolium chloride (TTC) staining was used to calculate the area of cerebral infarction and pathological examination of brain tissues.Results Compared with the model control group,the middle and high dosage of shenfu injection could obviously improve the nerve function and increase the percentage of cerebral infarction area (P ＜ 0.05);the high dosage group of shenfu injection could obviously decrease the whole blood viscosity (P ＜ 0.01);the middle and high dosage of shenfu injection could obviously reduce the level of MDA in rat brain tissue (P ＜ 0.01) while increasing the levels of SOD and GSH (P ＜0.01),finally could significantly improve the pathological changes of brain tissues such as mild swelling of nerve fibers,mild neuronal degeneration,inflammatory interstitial edema and inflammation.Conclusions Shenfu injection has obvious protective effect on cerebral ischemia reperfusion model in rats.
