The induction, purification, whole genome sequencing and comparative genome analysis of a novel staphylococcus haemolyticus bacteriophage IME-SA4
10.3760/cma.j.issn.1008-1372.2018.04.018
- VernacularTitle:一株新的溶血葡萄球菌溶原性噬菌体IME-SA4的诱导、全基因组测序和比较基因组分析
- Author:
Ping LIU
1
;
Fan PENG
;
Qi HU
;
YuLing TANG
;
Huaixing KANG
;
Yigang TONG
Author Information
1. 长沙市第一医院呼吸内科
- Keywords:
Staphylococcus haemolyticus;
Staphylococcus phages;
Virus activation;
Genome-wide association study
- From:
Journal of Chinese Physician
2018;20(4):546-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective To induce a novel temperate bacteriophage from staphylococcus haemolyticus strain SA98,observe the morphology and size,complete the whole genome sequencing,analyse the structure of genome and evolutionary relationship.Methods The mitomycin C was used to induce the temperate phage from staphylococcushaemolyticus strain SA98,the induced phage was observed by transmission electron microscopy after be concentrated and purified.The genome DNA was extracted and high through sequenced.The feature of whole genome and evolutionary relationship was analyzed.Results A temperate phage IME-SA4 was successfully induced from staphylococcus haemolyticus strain SA98.Transmission electron microscopy analysis indicated that IME-SA4 had an isometric head and a non-contractile long tail.The whole genome of IME-SA4 was long as 41 843 bp,and the whole genome blast result indicated IME-SA4 shared only 13％ homology with most related strain phiRS7.Conclusions A novel staphylococcus haemolyticus temperate phage with low homology with other staphylococcusphages was successfully induced from staphylococcus haemolyticus strain SA98.The research of its morphology,whole genome sequencing and comparative genome analysis make the foundation for further study of staphylococcus phages' properties and practical application.
