Detection of expressing of miR-103/KLF4 in A549 lung cancer cells and A549/DDP resistant cells and validation of the targeted relationship

VernacularTitle:miR-103/KLF4在肺癌A549细胞及耐药细胞中的表达检测及靶向关系验证
Author: Shenggang LIU ¹ ; Hongzhong YANG
Author Information

1. 410000,长沙市中心医院呼吸病诊疗中心
Keywords: MicroRNAs/ME; Kruppel-like transcription factors/ME; Lung neoplasms/PA/ME; Drug resistance,neoplasm
From: Journal of Chinese Physician 2018;20(3):402-407
CountryChina
Language:Chinese
Abstract: Objective To study the expression and targeted relationship of miR-103/KLF4 (Krüppel like factor) in A549 and resistant cell lines of lung cancer.Methods To culture the A549 cell lines and A549/DDP (cisplatin) resistant cell lines in vitro and detect survival rates by methyl thiazolyl tetrazolium (MTT) method.Real-time polymerase chain reaction (RT-PCR) and Western blotting were used to test the expression of miR-103/KLF4 of these cell lines.Dual-luciferase reporter gene experiment to detect the targeted relationship.Results A higher expression of miR-103 and a obvious lower expression of KLF4 were observed in A549/DDP resistant cell lines.MiR-103 target KLF4-3'UTR (3'untranslated regions) directly in lung cancer cells.Conclusions In A549/DDP resistant cells,miR-103 can regulate KLF4 at target.