Examination of the expressions of miR-221 in the human pulmonary fibrosis tissues and in A549 cells treated with TGFβ1
10.3760/cma.j.issn.1008-1372.2018.02.008
- VernacularTitle:miR-221在人肺纤维化组织和TGFβ1干预A549细胞中的表达变化
- Author:
Wei SHUAI
1
,
2
;
Qiong CHEN
;
Yichun WANG
Author Information
1. 400013 长沙,湖南省肿瘤医院重症医学科
2. 421001 湖南省衡阳市南华大学医学院
- Keywords:
MicroRNAs/ME;
Pulmonary fibrosis/PA;
Transforming growth factor beta1/AE;
Lung neoplasms/PA
- From:
Journal of Chinese Physician
2018;20(2):187-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of microRNA-221 (miR-221) in the human pulmonary fibrosis tissues and in adenocarcinoma(A549) cells treated with transforming growth factor beta 1 (TGFβ1).Methods Real time-PCR was used to detect the expressions of miR-221 in pathologically diag nosed as pulmonary fibrosis tissue and in A549 cells treated with TGFβ1.Results miR-221 in pulmonary fibrosis tissue was downregulated compared to normal tissues (P ＜ 0.05).The morphology of cells was changed from long spindle type to short, the number of cells was increased, and the adjacent cells were not connected closely.TGFβ1, N-cadherin, vimentin, α-smooth muscle actin (α-SMA), collagen Ⅰ, and collagen Ⅲ levels were higher, whereas E-cadherin level was lower in TGFβ1-treated group compared to the control group.miR-221 expression was downregulated in cells after TGFβ1 treatment (P ＜ 0.05).Conclusions miR-221 was downregulated in pulmonary fibrosis tissues and in A549 cells, which indicates that miR-221 may play an important role in the formation of pulmonary fibrosis.
