The negative-pressure wound therapy promotes wound healing by enhancing angiogenesis in granulation tissue and wound healing through suppression of NLRX1 through via the up-regulation of miR-195expression by suppressing NLRX1 expression upregulation
10.3760/cma.j.issn.1008-1372.2018.02.007
- VernacularTitle:负压创面影响肉芽血管生成过程中miR-195和NLRX1表达研究
- Author:
Xiaoxia CHEN
1
;
Ningning TANG
;
Huiqing XIE
;
Li QIAN
;
Feng HU
;
Chuwang WANG
;
Bin HE
;
Jiaqin XU
;
Zunhong LIANG
;
Jianda ZHOU
Author Information
1. 中南大学湘雅三医院整形外科
- Keywords:
Negative-pressure wound therapy;
MicroRNAs/ME;
Nod-like receptors/ME;
Granulation tissue/BS;
Granulation tissue/PA;
Neovascularization,pathologic
- From:
Journal of Chinese Physician
2018;20(2):183-186,190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and the relationship with angiogenesis of miR-195 and NLR family member X1 (NLRX1) in granulation tissue after negative-pressure wound treatment (NPWT).Methods Six patients were collected who received negative pressure treatment with refractory wound granulation.The levels of miR-195, NLRX1 mRNA and NLRX1 proteins were measured.The expression of NLRX1 and the micro-vascular density (MVD) of CD31 were detected by immunohistochemistry (IHC).Results MiR-195 and MVD were significantly higher in granulation tissue after 7 days negative pressure treatment (P＜0.05), and NLRX1 was significantly lower (P ＜0.05).In granulation tissue,the expression of miR-195 was negatively correlated with NLRX1 (r =-0.856, P ＜0.001), the expression of NLRX1 was negatively correlated with MVD (r =-0.618, P ＜0.05), and the expression of miR-195 was positively correlated with MVD (r =0.630, P ＜ 0.05).Conclusions Negative pressure wound therapy can promote the formation of granulation vessels and the wound healing.The therapeutic mechanism may inhibit the expression of NLRX1 and upregulate the expression of miR-195 to promote angiogenesis.
