Determination of Six Active Ingredients in Gualoupi Injection by Quantitative Analysis of Multi-components by Single Marker
- VernacularTitle:一测多评法同时测定瓜蒌皮注射液中6种有效成分含量
- Author:
Jingjing ZHANG
1
;
Qiong JIE
;
Yubing ZHU
Author Information
1. 南京医科大学附属南京医院(南京市第一医院)药学部 南京210006
- Keywords:
Gualoupi injection;
3,29-Dibenzoyl;
Vanillic acid;
Adenosine;
3,29-Dibenzoyl rarounitriol;
Quercitrin;
Cynaro-side;
Quantitative analysis of multi-components with a single marker
- From:
China Pharmacist
2018;21(10):1779-1782
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a method of quantitative analysis of multi-components by single marker (QAMS) for six active ingredients in Gualoupi injection. Methods: An HPLC method was used with a Waters Sunfire ODS C18column (250 mm×4. 6mm, 2. 5 μm), the mobile phase was methanol(A)-0. 2% glacial acetic acid solution(B) with gradient elution, the flow rate was 1. 0 ml· min-1,the detection wavelength was 254 nm,the column temperature was 30℃ and the injection volume was 20 μl. Using 3, 29-dibenzoyl rarounitriol as a reference, the relative correction factors among karounidiol, vanillic acid, adenosine, quercitrin and cynaro-side were detected by QAMS and their contents were calculated, and the results were compared with those of the external standard method. Results: The differences were not statistically significant between the calculated values of karounidiol, vanillic acid, adeno-sine, quercitrin and cynaroside and those measured by the external standard method (P>0. 05). Conclusion: QAMS can be used for the determination of 6 effective components in Gualoupi injection, and the result is accurate, simple and effective.
