HPLC Fingerprints of Compound Yinchen Granules Based on Detection Wavelength Switching Technology
- VernacularTitle:基于检测波长切换技术的复方茵陈颗粒HPLC指纹图谱研究
- Author:
Lixia SHEN
1
;
Yanwei FU
;
Songgang JI
;
Mingchun LI
;
Yanqin CHENG
Author Information
1. 解放军第401医院 山东青岛266071
- Keywords:
Compound Yinchen granules;
HPLC;
Fingerprints
- From:
China Pharmacist
2018;21(10):1769-1775
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the HPLC fingerprints of compound Yinchen granules. Methods: The column was Agilent SB-C18(250 mm×4. 6 mm, 5 μm) and the mobile phase was acetonitrile (A)-0. 2% phosphoric acid solution (B) with gradient elution at a flow rate of 1. 0 ml·min-1. The column temperature was 25℃. The detection wavelength switching technology was used in 180-mi-nute elution time. Results: The HPLC fingerprints of compound Yinchen granules were established. Twenty-two common peaks were confirmed, of which five peaks were identified and 18 peaks were assigned to each crude drug. The overall similarity of the fingerprints of 10 batches of samples was 0. 9 or more when compared with the control map. Conclusion: The fingerprints of compound Yinchen granules can provide reference for the overall quality control of compound Yinchen granules.
