Study on the HPLC Fingerprint of Caryophylli Flos.
- VernacularTitle:丁香高效液相指纹图谱研究
- Author:
Jiamin MENG
1
;
Hanmei JIANG
;
Jinqing LU
;
Han TU
;
Yushuo XIAO
Author Information
1. 湖北中医药大学 湖北省药用植物研发中心 武汉430065
- Keywords:
Caryophylli flos.;
Eugenol;
HPLC;
Fingerprint
- From:
China Pharmacist
2018;21(10):1762-1765,1775
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC fingerprint of Caryophylli flos. to provide reference for the overall quality evaluation of the herb. Methods: The fingerprint of Caryophylli flos. was established by HPLC, and eugenol was used as the reference peak for the exploration of HPLC conditions. The fingerprint of Caryophylli flos. was established with acetonitrile-0. 2% phosphoric acid as the mobile phase with gradient elution. Results: A total of 17 characteristic common peaks were obtained by HPLC analysis, and among them, five common constituents including eugenol, ferulic acid, quercetin, kaempferol and isorhamnetin were calibrated. The similari-ties of chromatographic fingerprint of Caryophylli flos. from 10 different origins were above 0. 900, which was in line with the basic re-quirement of fingerprint similarity evaluation. Conclusion: The study provides experimental basis for improving the quality evaluation system of Caryophylli flos. , and provides reference for a comprehensive quality assessment of Caryophylli flos. .
