Comparison of Different Purification Processes for the Extract of Herba Rhodiola
10.3969/j.issn.1008-049X.2018.06.049
- VernacularTitle:红景天提取物不同纯化工艺的比较
- Author:
Jianfeng DAI
1
;
Xinlei SHI
;
Wei WU
Author Information
1. 浙江中医药大学附属嘉兴中医院 浙江嘉兴314001
- Keywords:
Salidroside;
Purification;
Macroporous resin;
HPLC
- From:
China Pharmacist
2018;21(6):1123-1125
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the effects of different purification processes on the content of salidroside in the extract of Herba Rhodiola to provide reference for the large-scale production. Methods: Ethanol precipitation, clarifier precipitation and AB-8 macro-porous resin separation was respectively used to purify the extract of Herba Rhodiolae, and HPLC was used to determine the content of salidroside to screen the optimum purification process. Results: After the purification by AB-8 macroporous resin, the content of sali-droside was as high as 0. 272 5 mg·ml-1followed by that by ethanol precipitation (70% alcohol), and the clarifier precipitation lost more salidroside. Conclusion: The purification of salidroside by AB-8 macroporous resin separation is with high yield, good selectivi-ty, promising reproducibility and so on, which is suitable for the large-scale production of salidroside.
