Determination of Calcipotriol Ointment by High-speed Counter-current Chromatography
10.3969/j.issn.1008-049X.2018.06.047
- VernacularTitle:高速逆流色谱法测定卡泊三醇软膏的含量
- Author:
Nan YIN
1
;
Shuangtao DONG
;
Baoxia LI
Author Information
1. 辽宁中医药大学附属医院药剂科 沈阳110032
- Keywords:
High-speed counter-current chromatography;
Calcipotriol ointment;
Content determination
- From:
China Pharmacist
2018;21(6):1117-1119
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a new method for the determination of calcipotriol ointment by high-speed counter-current chro-matography(HSCCC). Methods: An HSCCC method was used coupled with two-phase solvent system consisting of petroleum benzine-ethanol -water(2: 1: 0. 5, v/v/v). The injection volume was 1. 0 ml and the detection wavelength was set at 265 nm. Results: The proposed method showed good linearity within the range of 4.02-40.20 μg·ml-1(r=0.999 4). The average recovery was 99.2%, and the RSD was 0. 4% (n=9) . Conclusion: The HSCCC method is simple and rapid, and suitable for the determination of calcipo-triol ointment.
