Determination of Benzyl Alcohol in Metamizole Sodium Injection by HPLC

VernacularTitle:HPLC法测定安乃近注射液中苯甲醇的含量
Author: Yi LIU ¹ ; Wen LAN ; Xiaoyi SHI
Author Information

1. 湖南省药品检验研究院长沙410001
Keywords: Benzyl alcohol; Metamizole sodium injection; HPLC; Determination
From: China Pharmacist 2018;21(6):1112-1114
CountryChina
Language:Chinese
Abstract: Objective: To establish an HPLC method to determine the content of benzyl alcohol in metamizole sodium injection. Methods: The determination was performed on an Xtimate C18(250 mm×4. 6 mm,5 μm)column. Phosphate buffer (dissolving 6. 0 g sodium dihydrogen phosphate in 1000 ml water, adding 1ml triethylamine, adjusting pH to 7. 0 with sodium hydroxide solution) -methanol (75: 25) was used as the mobile phase at a flow rate of 1. 0 ml·min-1. The detection wavelength was 254 nm. The column tamprture was 30 ℃ and the sample size was 5 μl. Results: The linear range of benzyl alcohol was 76. 88-269. 08 μg·ml-1( r=0. 998 5)with the average recovery of 98. 77% (RSD=0. 77% ,n=9). Conclusion: The method is accurate and reproducible for the content determination of benzyl alcohol in metamizole sodium injection.