Improvement of the Quality Standard for Fuzheng Capsules
10.3969/j.issn.1008-049X.2018.06.037
- VernacularTitle:扶正胶囊质量标准的提高
- Author:
Minna YAO
1
;
Deqiang LIU
;
Ning MA
;
Wei ZHANG
;
Ruili LI
;
Zhifu YANG
;
Aidong WEN
Author Information
1. 第四军医大学西京医院药剂科 西安710032
- Keywords:
Fuzheng capsules;
Quality standard;
HPLC;
TLC;
Microbial limit test
- From:
China Pharmacist
2018;21(6):1086-1089
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the quality standard for Fuzheng capsules. Methods: TLC was adopted to identify Astragali Radix and Glycyrrhiza uralensis. The method validation for Fuzheng capsules was conducted by microbial limit test as described in the appendixes of Chinese Pharmacopeia (2015 edition). The content of epimedii in Epimedium brevicornu was determined by HPLC. The chromatographic separation was carried out on an Agilent TC-C18(2) (250 mm×4. 6 mm, 5 μm) column. The mobile phase consis-ted of acetonitrile-water( 30: 70) with gradient elution at a flow rate of 1. 0 ml·min-1,and the injection volume was 5 μl. The detec-tion wavelength was 270 nm. Results: The spots in TLC were clear without any interference. The methods of plating and direct inocu-lation could be used for the microbial limit test. The linear range was 0.101-1.008 μg (r =0.999 7). The average recovery was 99. 36% with the RSD of 0. 81% (n=6). Conclusion: The method is simple with high specificity and good repeatability, which can be used as the quality control method for Fuzheng capsules.
