Extraction and Adsorption Separation of Chlorogenic Acid in Lonicera Macranthoides by a Blitzkrieg Ex-tractor and a Macroporous Resin
10.3969/j.issn.1008-049X.2018.06.012
- VernacularTitle:闪式提取-大孔树脂吸附分离山银花中绿原酸的工艺研究
- Author:
Guangyu CHEN
1
,
2
;
Fahuan GE
;
Qun HE
;
Mengzhou XIE
Author Information
1. 湖南中医药大学 湖南省药食同源功能性食品工程技术研究中心 长沙410208
2. 中山大学生命科学学院
- Keywords:
Lonicera macranthoides;
Chlorogenic acid;
Blitzkrieg extractor;
Macroporous resin adsorption separation;
ADS-7 Macroporous resin
- From:
China Pharmacist
2018;21(6):997-1002
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the optimal extraction process by a blitzkrieg extractor and the adsorption separation by macro-porous resin for chlorogenic acid in Lonicera macranthoides. Methods: The transfer rate of chlorogenic acid was used as the main as-sessment index, the response surface test was adopted to optimize the main influencing factors in the extraction process by a blitzkrieg extractor for chlorogenic acid in Lonicera macranthoides. The transfer rate and the product purity of chlorogenic acid were used as the main assessment indices, the water extract solution of Lonicera macranthoides was used as the raw material, single factor test was adopt-ed to optimize the main influencing factors in the adsorption separation process by a macroporous resin for chlorogenic acid in Lonicera macranthoides. Results: The optimum conditions of the extraction process by a blitzkrieg extractor and the adsorption separation by a macroporous resin for chlorogenic acid in Lonicera macranthoides were as follows: the solid-liquid ratio was 1 : 23, the extraction time was 4. 7 min, the rotating speed was 6 000 r·min-1, the type of macroporous resin was ADS-7, the amount ratio of medicinal materi-als to macroporous resin was 1: 5, the flow rate of sample solution was 1 BV·h-1, the macroporous resin column was eluted by 1 BV water at the speed of 1 BV·h-1followed by 6 BV 20% ethanol solution at the speed of 2 BV·h-1, and the eluent of 20% ethanol so-lution was collected. The average transfer rate of chlorogenic acid was 90. 12% (RSD=1. 33% ) with the purity of 50. 30% (RSD=2. 19% ). Conclusion: A new route of the extraction process by a blitzkrieg extractor and the adsorption separation by a macroporous resin for chlorogenic acid in Lonicera macranthoides has established through the optimization and verification experiments. The process with high transfer rate of chlorogenic acid (the purity of chlorogenic acid product was over 50% ) is fast, and the solvent is healthy and easy to recycle.
