Effects of Pulchinenoside at Different Concentrations on the Proliferation and Migration of Squamous Cell Carcinoma Cells and Calpain 1, E-cadherin, N-cadherin Protein
10.3969/j.issn.1008-049X.2018.06.009
- VernacularTitle:不同浓度白头翁皂苷对口腔鳞癌细胞增殖、迁移和钙蛋白酶1、E-cadherin、N-cadherin蛋白的影响
- Author:
Weijuan PAN
1
;
Ling DING
Author Information
1. 上海交通大学医学院附属仁济医院口腔科 上海201100
- Keywords:
Pulchinenoside;
Oral squamous cell carcinoma;
Proliferation;
Migration;
Calpain 1
- From:
China Pharmacist
2018;21(6):984-988
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the influences of pulchinenoside on the proliferation and migration of oral squamous cell carcino-ma cells and the expression of calpain 1 ( calpain 1). Methods: The human oral squamous cell carcinoma CAL27 cells were treated with pulchinenoside at the concentrations of 0, 1. 0,2. 0, 4. 0, 8. 0, 12. 0 mg·L-1. MTT method was used to detect the effect of pul-chinenoside on the proliferation of CAL27 cells; the effects of pulchinenoside on the proliferation and migration of CAL27 cells were de-tected by cell scratch test and Transwell experiment; the expression changes of calpain 1, E-cadherin and N-cadherin protein in CAL27 cells were detected by Western blot. Results: MTT results showed that, compared with the control group, the inhibitory rate of pul-chinenoside for the proliferation of CAL27 cells increased significantly (P<0. 05) in a time- and dose-dependent manner. The light microscope results showed that the CAL27 cells morphology changed from the long spindle shape to the cobblestone like epithelioid after the 48-hour treatment with 12. 0 mg·L-1pulchinenoside. Cell scratch test results showed that with the increase of pulchinenoside con-centration, the CAL27 cell migration ability gradually weakened. Transwell experimental results show that with the increase of pul-chinenoside concentration, the CAL27 cell migration and invasion abilities decreased gradually (P <0.05). Western blot results showed that with the increase of the concentration of pulchinenoside, the expression levels of calpain 1 and N-cadherin protein in CAL27 cells decreased gradually (P<0. 05), the expression level of E-cadherin protein increased gradually (P>0. 05). Conclusion:Pulchinenoside can inhibit the proliferation, migration and invasion of oral squamous cell carcinoma CAL27 cells, and its mechanism may be related to the down-regulation of calpain 1 and N-cadherin expressions and up-regulation of E-cadherin expression.
