Determination of Borneol and Iso-borneol in Niuhuang Qingxin Pills ( Prescription of the Bureau) by GC
10.3969/j.issn.1008-049X.2018.06.008
- VernacularTitle:气相色谱法测定牛黄清心丸(局方)中龙脑和异龙脑含量
- Author:
Lixing NIE
1
;
Zhong DAI
;
Shuangcheng MA
Author Information
1. 中国食品药品检定研究院 北京100050
- Keywords:
Niuhuang Qingxin pills (prescription of the bureau);
Borneol;
Iso-borneol;
Determination;
GC
- From:
China Pharmacist
2018;21(6):981-983
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a GC method for the determination of borneol and isoborneol in Niuhuang Qingxin pills ( pre-scription of the bureau). Methods: A capillary column HP-INNOWAX(30 m×0. 25 mm,0. 25 μm) was used and the column tem-perature was kept at 110 ℃. The temperature of the inlet and the FID was 200 ℃ and 230 ℃, respectively. The carrier gas was N2 and the flow rate of the carrier gas was 1. 8 ml·min-1. The split ratio was 10: 1, and the injection volume was 1 μl. Results: The linear response range of borneol and isoborneol was 0. 01-5. 09 μg·ml-1(r=0. 999 5) and 0. 01-5. 03 μg·ml-1(r=0. 999 1), re-spectively. And the average recovery was 99. 34% and 99. 24% with the RSD of 0. 59% and 0. 62% , respectively (n=6). Conclu-sion: The proposed method is accurate, sensitive and simple, and can be used for the quality control of Niuhuang Qingxin pills (pre-scription of the bureau).
