UPLC Typical Chromatogram of Jinji Pills
10.3969/j.issn.1008-049X.2018.06.005
- VernacularTitle:金鸡丸UPLC特征图谱研究
- Author:
Xiaoyan HUANG
1
;
Yinhong WANG
;
Jiangnan LUO
;
Ruilian LI
;
Zhenyu PAN
Author Information
1. 湖南省药品检验研究院 长沙410001
- Keywords:
Jinji pills;
UPLC typical chromatogram;
Berberine hydrochloride;
Quality evaluation
- From:
China Pharmacist
2018;21(6):970-972
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the UPLC typical chromatogram and evaluate the quality of Jinji pills. Methods: The UPLC typi-cal chromatogram was performed on an Agilent proshell 120 C18column (150 mm×4. 6 mm,2. 7 μm) with gradient elution by the mobile phase of acetonitrile-0. 1% phosphoric acid aqueous solution system at a flow rate of 0. 8 ml·min-1. The detection wavelength was 240 nm. Results: The UPLC typical chromatogram included 15 common peaks when taking berberine hydrochloride as the reference peak, and 7 of them were identified by the comparison with the reference substances. Conclusion: The established methods have high specificity and good repeatability, and can be used for the quality control of the product.
