Effects of Liraglutide on Oxidative Stress and Endoplasmic Reticulum Stress of H9c2 Myocardial Cells Cul-tured in High Glucose

VernacularTitle:利拉鲁肽对高糖培养的H9c2心肌细胞氧化应激和内质网应激的影响
Author: Tuohua MAO ¹ ; Dan LIU ; Jing LI ; Ling GAO ; Qing ZHANG
Author Information

1. 武汉大学人民医院内分泌科武汉430060
Keywords: Liraglutide; Oxidative stress; Endoplasmic reticulum stress; Diabetic cardiomyopathy
From: China Pharmacist 2018;21(6):956-959
CountryChina
Language:Chinese
Abstract: Objective: To investigate the effects of liraglutide on high glucose induced oxidative stress and endoplasmic reticulum stress of H9c2myocardial cells. Methods: Liraglutide and N- scavenger N-acetylcysteine ( NAC, a reactive oxygen species ( ROS) scavenger) were added to the H9c2 cells culture solution for 24h. The ROS levels were detected by flow cytometry, and Western blot was used to detect the expression levels of glucose regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP). GRP78 and CHOP were the endoplasmic reticulum stress protein markers. Results: Compared with the control group, the ROS, GRP78 and CHOP protein levels significantly increased in the high glucose group (P<0. 01). Compared with the high glucose group, liraglutide significantly reduced the overproduction of ROS (P<0. 01). Liraglutide also decreased the protein expression levels of GRP78 and CHOP (P<0. 05). Conclusion: Liraglutide can inhibit high glucose induced oxidative stress and endoplasmic reticulum stress of H9c2 cells.