Determination of Six Index Components in Xiaoyao Pills by Quantitative Analysis of Multi-components by Single Marker
- VernacularTitle:一测多评法同时测定逍遥丸中6种指标性成分的研究
- Author:
Yonghua DONG
1
;
Chuang YIN
Author Information
1. 皖北煤电集团总医院药剂科 安徽宿州234000
- Keywords:
Quantitative analysis of multi-components by single marker;
Xiaoyao pills;
Chlorogenic acid;
Paeoniflori;
Liquiritin;
Ferulic acid;
Glycyrrhizic acid;
Ligustilide
- From:
China Pharmacist
2018;21(5):930-933
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a method of quantitative analysis of multi-components by single marker (QAMS) for six active constituents in Xiaoyao pills. Methods:Using glycyrrhizic acid as the reference, an HPLC method with a Kromasil C18 column (250 mm ×4.6 mm,5 μm) was applied,the mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B) with gradient elution (0-10 min,5 % A;10-25 min,5 % →20 % A;20-70 min,15 % →60 % A and 40-60 min,60 % A). The detection wavelength was 280nm,the column temperature was 35℃ and the injection volume was 10 μl. The relative correction factors among the six index components were detected by QAMS. The contents of the six index components were determined by an external standard method and QAMS to compare the results obtained from the two different methods and verify the practicability and stability of QAMS. Results:The established QAMS was used to determine the six index components in Xiaoyao pills,and totally 6 batches of Xiaoyao pills were determined.There were no significant differences in the calculated values and the determined ones (P > 0.05). Conclusion:The QAMS method is simple,effective and accurate in determining the contents of the six index components in Xiaoyao pills,which can be used for the quality control of Xiaoyao pills and provide reference for the further study.
