Simultaneous Determination of Taurine and Aminocaproic Acid in Compound Taurine Eye Drops by High Performance Liquid Chromatography-Refractive Index Detector
- VernacularTitle:HPLC-RID法同时测定复方牛磺酸滴眼液中牛磺酸和氨基己酸的含量
- Author:
Shuhong WU
1
;
Lixia WANG
Author Information
1. 北京市东城区食品药品安全监控中心 北京100050
- Keywords:
Compound taurine eye drops;
Refractive index detector;
HPLC-RID;
Content determination;
Taurine;
Aminocaproic acid
- From:
China Pharmacist
2018;21(5):919-922
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC-RID method for the simultaneous determination of taurine and aminocaproic acid in compound taurine eye drops. Methods:A Waters NH2column(250 mm ×4.6 mm,5 μm) was used, the mobile phase was water-acetonitrile (40:60) at a flow rate of 0.8 ml·min-1, the column temperature was 35.0℃, and the temperatures of the detector reference cell and the sample cell were both set at 35.0℃,The injection volume was 20 μl. Results:The linear ranges of taurine and aminocaproic acid were 0.05-2.00 mg·ml-1(r = 0.999 9). The average recovery was 98.57% (RSD = 1.07%,n= 9) and 100.69% (RSD =0.75%,n =9),respectively. Conclusion:The established method is accurate,simple and rapid, and suitable for the quality control of compound taurine eye drops.
