Study on the HPLC Fingerprint of Yiqing Granules
- VernacularTitle:一清颗粒HPLC指纹图谱研究
- Author:
Mengmin WANG
1
;
Zhi TAO
;
Yan ZHANG
Author Information
1. 武汉黄陂区人民医院盘龙分院 武汉430300
- Keywords:
Yiqing granules;
Fingerprint;
Baicalin;
Berberine hydrochloride;
Rhein;
Aloe-emodin;
Wogonin;
Emodin;
Chrysophanol
- From:
China Pharmacist
2018;21(5):836-839
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC fingerprint of Yiqing granules to provide reference for the effective quality control. Methods:The analysis was carried out on an analytical Agilent ZORBAX SB-C18(250 mm×4.6 mm,5 μm)column with gradient elution by acetonitrile(A)-0.2% phosphoric acid solution(adjusting pH to 3.0 with triethylamine,B)(0-5 min:10% A→20%;5-20 min:20% A→30% A;20-25 min:30% A→45% A;25-35 min:45% A;35-45 min:45% A→80% A;45-50 min:80% A)at the detection wavelength of 254 nm and the flow rate of 1.0 ml·min-1,and the column temperature was 35 ℃. Twelve batches of Yiqing granules were determined by HPLC and a common mode of fingerprint was established. Results:There were 22 common peaks in the fingerprints of the twelve batches of Yiqing granules,and seven of them were identified. The similarities of fingerprints of the twelve batches of Yiqing granules were over 0.990 suggesting good reproducibility. Conclusion:The developed method is accurate and feasible,and can be used for the quality control of Yiqing granules.
