Study on the Quality Control Method for Ganoderma Lucidum Using Quantitative Analysis of Multi-components by Single Marker
- VernacularTitle:灵芝一测多评质量控制方法的研究
- Author:
Lei FAN
1
;
Qinming CHEN
;
Zhangjin CHEN
;
Min LIU
;
Huali YU
Author Information
1. 丽水市食品药品与质量技术检验检测院 浙江丽水323000
- Keywords:
Ganoderma lucidum;
Quantitative analysis of multi-components by single marker;
Relative correction factor;
Triterpenoid
- From:
China Pharmacist
2018;21(5):828-832
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an analysis method for determining the contents of 7 components including ganoderic acid C2, ganoderic acid B,ganoderic acid G,ganoderic acid A,lucidenic acid A,ganoderma D and ganoderic acid F in ganoderma by QAMS. Methods:An HPLC method was used. The column was WatersX-bridge C18(250 mm × 4.6 mm, 5 μm), the mobile phase was acetonitrile(A)-1% acetic acid(B) (28:72) with gradient elution(0-30 min:A-28% to 38%;30-45 min:A-38% to 55%) at a flow rate of 1.0 ml·min-1,the column temperature was 30℃, and the detection wavelength was 254 nm. Ganoderic acid A was used as the internal reference, the correction factors of ganoderic acid C2, ganoderic acid B, ganoderic acid G, lucidenic acid A,ganoderma D and ganoderic acid F were calculated,and the contents of the 7 components were calculated by an external standard method to verify the feasibility and applicability of QAMS. Results:The relative correction factors of multiple assessments were reproducible,and the relative deviation of the contents of 6 components in 16 batches of Ganoderma lucidum was less than 2% when compared with that of the external standard method. Conclusion:QAMS is accurate and reliable in the quality evaluation of Ganoderma lucidum.
