Effect of Sinomenine Hydrochloride on TLR4/NF-κB Pathway in Mice with Experimental Colitis
- VernacularTitle:盐酸青藤碱对实验性结肠炎小鼠TLR4/NF-κB信号通路的影响
- Author:
Liang TIAN
1
;
Jie XUE
;
Lili LIU
;
Yi YU
;
Yingjun FU
Author Information
1. 上海德济医院青岛大学第九临床医学院 上海200331
- Keywords:
Sinomenine;
Colitis;
TLR4/NF-κB pathway
- From:
China Pharmacist
2018;21(5):797-801
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of sinomenine hydrochloride on dextran sulfate sodium(DSS) induced experimental colitis in mice. Methods:The colitis model of BALB/c mice was established with DSS. The mice were randomly divided into six groups (n = 6):the normal control group and the model group(giving normal saline,0.2 ml),salicylazosulfapyridine treatment group (100 mg·kg-1) and sinomenine treatment groups (30,90,270 mg·kg-1). After continuous administration for 10 d,the disease activity index (DAI) and the tissue damage index in each group were evaluated. The mouse colons of TLR4 and Myd88 were detected using Western-blotting, and the expression of NF-κB in the colon tissues was detected using immunohistochemical method. Results:The DAI and the tissue damage index, and the expressions of TLR4, Myd88 and NF-κB in the colon tissues were significantly higher in the model group than those in the normal group (P < 0.01). Sulfasalazine treatment group and sinomenine at low,medium and high dose groups could reduce the DAI and the tissue damage score,and the expressions of TLR4, Myd88 and NF-κB in the colon tissues (P < 0.01) in a concentration-dependent manner. There was no significant difference between salicylazosulfapyridine treatment group and sinomenine at medium/high dose groups. Conclusion:Sinomenine reduces the expression of TLR4,Myd88 and NF-κB in mice with experimental colitis by regulating the TLR4/NF-k B signaling pathway to play a therapeutic role.
